Bovine serum albumin detection by using molecularly imprinted surface plasmon resonance sensors

Molecular imprinted polymers (MIP) have key-lock pattern binding properties specific to the size and shape of target molecules. In this study, we prepared detection platforms based on a molecularly surface plasmon resonance (SPR) sensor that can detect bovine serum albumin (BSA) sensitively, selectively, quickly, in real time. The polymeric film SPR by molecular imprinting method was obtained selecting N-methacryloyl-(L)-glutamic acid molecule as suitable functional monomer using ultraviolet polymerization. Three different methods, such epitope, bulk, were used examine efficiency. Real-time measurements performed with BSA provide linearity concentration range from 0.10 7.50 nM indicate limit value 0.015 nM. Furthermore, selectivity experiments, where transferrin hemoglobin chosen competitor agents. Overall, epitope approach has been found be highly selective sensitive for albumin. To statistically assess reusability sensor, intraday experiments tested three times five replicates. RSD% less than